Peptidyl-Lys Metalloendopeptidase-catalyzed 18O Labeling for Comparative Proteomics
نویسندگان
چکیده
منابع مشابه
Proteolytic 18O labeling for comparative proteomics: evaluation of endoprotease Glu-C as the catalytic agent.
Recently, proteolytic 18O labeling has been demonstrated as a promising strategy for comparative proteomic studies (Yao, X.; Freas, A.; Ramirez, J.; Demirev, P. A.; Fenselau, C. Anal. Chem. 2001, 73, 2836-42). In this approach, protein mixtures are digested in parallel in H216O and H218O and the ratios of isotopically distinct peptide products are measured by mass spectrometry. In the initial r...
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Quantitative changes in cytochrome P450 (CYP) proteins involved in drug metabolism as a consequence of drug treatment are important parameters in predicting the fates and pharmacological consequences of xenobiotics and drugs. In this study we undertook comparative P450 proteomics using liver from control and 1,4-bis-2-(3,5-dichloropyridyloxybenzene) (TCPOBOP)-dosed mice. The method involved sep...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2005
ISSN: 1535-9476
DOI: 10.1074/mcp.m500150-mcp200